resumo
Enhancement of Raman signals of the nucleobase adenine on an Ag based composite was studied using the 1064 nm laser line. The composite comprise emulsions of Ag nanoparticles encapsulated in poly(t-butylacrylate) (PtBA) beads that act as substrate for the Surface-Enhanced Raman Scattering (SERS) of adenine. For this system, Raman enhancement was observed for the ring-stretching vibrational mode of adenine after aggregation of the Ag/poly(t-butylacrylate) emulsion and isolation of the solid composite. This is a convenient and alternative analytical approach to SERS monitoring of solutions of adenine over the more common use of pure Ag colloids. As a consequence, this research contributes to develop innovative studies on DNA fragments using polymeric platforms that can act as highly sensitive SERS substrates. (C) 2012 Elsevier B.V. All rights reserved.
palavras-chave
ENHANCED RAMAN-SPECTROSCOPY; DNA BASES; SCATTERING; NANOPARTICLES; BIOANALYSIS; FILMS; PH
categoria
Spectroscopy
autores
Pinheiro, PC; Fateixa, S; Nogueira, HIS; Trindade, T
nossos autores
Helena Isabel Seguro Nogueira
Professor Auxiliar
Paula Pinheiro
Bolseiro de investigação
Sara Isabel Augusto Fateixa
Investigador
Tito Trindade
Professor CatedráticoProjectos
Magnetotransduction: development of magnetic nanoparticles-viral vector complexes for therapeutic gene delivery in the senile brain (EULANEST European-Latin American Network for Science and Technology) (NEURONANO)
Centre for Research in Ceramics and Composite Materials - CICECO (LA0011: 2011-2012) (PEst-C/CTM/LA0011/2011)
agradecimentos
We thank Fundacao para a Ciencia e Tecnologia (FCT/FEDER) for the grant SFRH/BD/66460/2009 (S. Fateixa) and projects ERA-Eula/0003/2009 (P.C. Pinheiro), and Pest-C/CTM/LA0011/2011, FSE and POPH.