resumo
An integrated methodology for the identification and quantification of linoleic acid hydroperoxides (HPODEs) mixtures, obtained by the Fenton's reaction and by enzymatic oxidation, is reported. Unambiguous identification of the HPODEs formed (13-hydroperoxy-(9Z,11E)-octadecadienoic acid, 13-hydroperoxy-(9E,11E)-octadecadienoic acid and 9-hydroperoxyoctadecadienoic acid) was performed by HPLC-MS/MS analysis, while quantification was carried out by HPLC-UV using an external calibration, based on the collection of chromatographic peaks, and measuring of total hydroperoxides by ferrous oxidation-xylenol orange (FOX) method. (C) 2011 Elsevier B.V. All rights reserved.
palavras-chave
MASS-SPECTROMETRY; RENEWABLE RESOURCE; OXIDATION; PRODUCTS; OIL; CHEMISTRY; TISSUES; CHAIN; TIME
categoria
Agriculture
autores
Villaverde, JJ; Santos, SAO; Simoes, MMQ; Neto, CP; Domingues, MRM; Silvestre, AJD
nossos autores
Grupos
4 - biorrefinarias, materiais de origem biológica e reciclagem
5 - materiais biomédicos e biomiméticos
agradecimentos
FCT and EU are acknowledged for financial support of the ERA-NOEL Project (Novel enzyme tools for production of functional oleochemicals from unsaturated lipids - ERA-IB/BIO/0001/2008), which also funds J.J. Villaverde Postdoctoral Grant.