abstract
BACKGROUND: A sequential batch reactor (SBR) was used for eucalypt kraft pulp effluent treatment with Trametes versicolor. A 2(3) full factorial design and response surface methodology were applied to optimise the batch fermentation conditions. Effluent concentration, culture medium and inoculum age were the factors selected for this study in order to optimise the reduction of chemical oxygen demand (COD). RESULTS: The presence of Trametes Defined Medium (TDM) in the fermentation was required to obtain a significant COD reduction. Experiments in the batch reactor confirmed, in general, the predicted results of optimisation developed from Erlenmeyer batch assays. The T. versicolor culture remained active during 42 days of study in the SBR, providing approximately 80%, of COD reduction. CONCLUSION: Trametes versicolor may be considered as very promising for the biological treatment of effluents from kraft pulp mills in an SBR system instead of the activated sludge mixed cultures traditionally used. (C) 2008 Society of Chemical Industry
keywords
PAPER-MILL EFFLUENT; LACCASE INDUCTION; DECOLORIZATION; WASTEWATERS; PEROXIDASE; REMOVAL; LIGNIN; SYSTEM
subject category
Biotechnology & Applied Microbiology; Chemistry; Engineering
authors
Xavier, AMRB; Tavares, APM; Agapito, MSM; Evtuguin, DV
our authors
acknowledgements
The authors acknowledge the Fundacao para Ciencia e Tecnologia (FCT), Portugal for financial support of this work (project POCTI/EQU/48489/2002) and Portucel - Empresa Produtora de Pasta e Papel, SA, Cacia, Portugal for helpful collaboration. APM Tavares acknowledges her Post-Doc scholarship provided by FCT (SFRH/BPD/22697/2005).