resumo
The precursor Ru([9]aneS(3))(DMSO)Cl-2 (1), where [9] aneS3 represents 1,4,7-trithiacyclononane, was dissolved in acetonitrile at 50 degrees C and stirred for several hours to assess the solvent's ability to coordinate to 1. A bis(acetonitrile) Ru(III) compound (2) crystallized as solid yellow needles upon slow evaporation of the reaction solution at room temperature. The structure of 2 was investigated in the solid state by FT-IR and single-crystal diffraction, showing it to be [Ru(III)([9]aneS(3))(NCMe)(2)Cl]Cl-2 center dot 2H(2)O (2). To assess the biological action of 2, growth-inhibition tests were carried by the MTT assay on a human tumor cell line of interest to our group, the osteosarcoma MG-63 line. Results have shown that the complex requires concentrations above 200 mu mol L-1 at 72 h of incubation to display cytotoxic action on this cell line.
palavras-chave
DNA-BINDING; NAMI-A; DRUGS
categoria
Chemistry
autores
Marques, J; Fernandes, JA; Paz, FAA; Marques, MPM; Braga, SS
nossos autores
Filipe Alexandre Almeida Paz
Investigador Principal
José Alberto Pires Fernandes
Bolseiro de pós-Doutoramento
Susana Isabel Fonseca de Almeida Santos Braga
Investigadoragradecimentos
We are grateful to the Fundacao para a Ciencia e a Tecnologia (FCT), Orcamento de Estado (OE), and Fundo Europeu de Desenvolvimento Regional (FEDER) through the program COMPETE (Programa Operacional Factores de Competitividade) for their general financial support and for specific funding toward the purchase of the single-crystal diffractometer. The FCT and the European Social Fund, through the Programma Operacional Potencial Humano (POPH), are acknowledged for a PhD grant to J.M. (SFRH/BD/44791/2008), and for a postdoc grant to J.A.F. (SFRH/BPD/63736/2009).